Journal: International Journal of Oncology

Article Title: FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

doi: 10.3892/ijo.2015.2940

Figure Lengend Snippet: FK-3000 and sinococuline isolated from S. delavayi Diels. Fraction 3 from the initial S. delavayi Diels. extract chromatography was further purified by C18 HPLC. This yielded sinococuline at R t 36.01 min and FK-3000 at R t 82.14 min.

Article Snippet: Attached cells were treated with 5.0 μg/ml FK-3000 and incubated for 120 min in a Lab-Tek ® II Chamber Slide™ system (Nalge Nunc International).

Techniques: Isolation, Chromatography, Purification

Journal: International Journal of Oncology

Article Title: FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

doi: 10.3892/ijo.2015.2940

Figure Lengend Snippet: FK-3000 increased MDA-MB-231 cell apoptosis in a dose- and time-dependent manner. In the 5.0 μg/ml FK-3000-treated cells, the apoptotic cell percentage increased from 12.97% at 24 h to 37.69% at 48 h. Following 48-h incubation, the percentage of apoptotic cells was 11.34% in control cells and 37.69% in 5.0 μg/ml FK-3000-treated cells.

Article Snippet: Attached cells were treated with 5.0 μg/ml FK-3000 and incubated for 120 min in a Lab-Tek ® II Chamber Slide™ system (Nalge Nunc International).

Techniques: Incubation

Journal: International Journal of Oncology

Article Title: FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

doi: 10.3892/ijo.2015.2940

Figure Lengend Snippet: FK-3000 effectively blocks NF-κB translocation from cytoplasm to nucleus. (A) In untreated MDA-MB-231 cells NF-κB p65 proteins [green color (b)] are localized primarily in the nucleus [blue color (a)]. The merged image (c) confirms the NF-κB p65 protein localization. (B) In the 5.0 μg/ml FK-3000-treated cells, NF-κB p65 proteins (b) were almost exclusively localized to the cytoplasm (a). The merged image (c) confirms that the NF-κB p65 proteins are not in the cell nucleus.

Article Snippet: Attached cells were treated with 5.0 μg/ml FK-3000 and incubated for 120 min in a Lab-Tek ® II Chamber Slide™ system (Nalge Nunc International).

Techniques: Translocation Assay

Journal: International Journal of Oncology

Article Title: FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

doi: 10.3892/ijo.2015.2940

Figure Lengend Snippet: FK-3000 reduced NF-κB phosphorylation levels and COX-2 expression. In MDA-MB-231 cells treated with 5.0 μg/ml FK-3000 for 120 min, NF-κB phosphorylation decreased to nearly undetectable levels compared with the 0.5 μg/ml treated cells at 120 min or the 5.0 μg/ml treated cells at 60–90 min. FK-3000 suppressed COX-2 protein expression in a dose- and time-dependent manner.

Article Snippet: Attached cells were treated with 5.0 μg/ml FK-3000 and incubated for 120 min in a Lab-Tek ® II Chamber Slide™ system (Nalge Nunc International).

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

doi: 10.3892/ijo.2015.2940

Figure Lengend Snippet: FK-3000 inhibits tumor growth in an MDA-MB-231 xenografted mouse model. Tumor sizes in mice treated with vehicle alone (0.1% DMSO), FK-3000 (1 mg/kg body weight/day), Taxol (10 mg/kg body weight/week), or FK-3000 and Taxol combined. From day 15 onwards, tumor volumes of the drug-treated groups were significantly different from the control. Values represent the mean ± standard deviation. * p<0.1; ** p<0.05, compared to the control, as determined by Tukey’s range (HSD) test.

Article Snippet: Attached cells were treated with 5.0 μg/ml FK-3000 and incubated for 120 min in a Lab-Tek ® II Chamber Slide™ system (Nalge Nunc International).

Techniques: Standard Deviation

Journal: Stem Cells Translational Medicine

Article Title: Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells

doi: 10.5966/sctm.2015-0324

Figure Lengend Snippet: HOXB4 activation had no significant effect on the maturation of pluripotent stem cell-derived erythroid cells. High-performance liquid chromatography analysis of cell lysates generated at day 24 of the differentiation protocol from control (H1) and HOXB4-activated cells [in the presence (+T) and absence (−T) of tamoxifen] (A) . The amount of the different globins detected as a proportion of the total globin content was calculated in six independent experiments (B) . Adult peripheral blood and fetal blood were included for comparison. Enucleation of control and HOXB4-expressing human embryonic stem cells (hESCs) in the presence (+T) and absence (−T) of tamoxifen was assessed by flow cytometry using the nuclear Syto Red stain and the erythroid marker, CD235a (C) . A representative histogram of SYTO Red staining of control enucleated peripheral blood samples (blue), nucleated mononuclear cells (green), and hESCs after HOXB4 activation (orange) are shown together with the unstained control hESCs (D) . Abbreviation: HuESC, human embryonic stem cell.

Article Snippet: Globin chain separation was performed by injecting 10 μl of the supernatant onto a 1.0 × 250-mm C4 column (Phenomenex, Macclesfield, U.K., http://www.phenomenex.com ) with a 42%–56% linear gradient between mixtures of 0.1% TFA in water and 0.1% TFA in acetonitrile at a flow rate of 0.05 ml per minute for 50 minutes on a HPLC Ultimate 3000 system (Dionex, Thermo Fisher Scientific Life Sciences).

Techniques: Activation Assay, Derivative Assay, High Performance Liquid Chromatography, Generated, Control, Comparison, Expressing, Flow Cytometry, Staining, Marker

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

Article Title: A draft genome of field pennycress ( Thlaspi arvense ) provides tools for the domestication of a new winter biofuel crop

doi: 10.1093/dnares/dsu045

Figure Lengend Snippet: Genome sequencing, assembly, and annotation statistics

Article Snippet: Illumina HiSeq 2000 (2 × 50 bp Mate Pair) 2, 3, 5 kb inserts , 209,815,249 , 9,550.66.

Techniques: Sequencing